The learning and memory behavior detection method, test method

Experimental principle:

On the basis of animal behavior, rodent animal in the open field, most of the time will stay on the wall or the corner. Be on a pedestal, will jump by instinct, to avoid the wall or the corner. The bottom of the jumping apparatus can be energized, placed above an insulating board. Animal shock in training, jumped on the platform to avoid the shock, resulting in memory. Animal memory decline, performance for the first time is shortened from jumping jump latency, number of jump increase. When the animal's memory is enhanced, the latency of the jump is prolonged, and the electric shock frequency is obviously reduced.

Experimental procedure:

1 adaptation: put the animal on the platform, so that the animals in the box up and down for 2-3 minutes, and then put the animal back into the box. Or every time to adapt to 10 seconds, a total of 3 times, 30 minutes interval. The latter makes animals more fully adapted to the gestures of the subjects and the whole process of the experiment.

2 training: can be adapted immediately after the adaptation, but also in the 24 hours after the adaptation.

The animal once again put on the platform, while opening the electrical stimulator,

The copper grid power supply. Animals because in the box to adapt, almost immediately jumped off the platform, limbs and immediately suffered shock. Due to the painful electric shocks, jumping up and down on the animal, occasionally to inadvertently jumped back to the platform, immediately jumped platform, so repeated several times.

Recording index: the detection technique of learning and memory behavior of small animals: a method of avoidance

1) animals from the platform to jump off the platform to the incubation period;

2) the number of repeated step down the number of errors, can represent the animal's academic performance;

3 weight test: 24 hours after training, re test, once again put the animal on the platform to observe the situation of animals to step down.

Record index:

Animals put on the platform to jump off the platform after the incubation period, 5 minutes to jump off the platform error times, this is the animal's memory performance.